Magic-BLAST — NCBI aligner for mapping large next-generation RNA or DNA sequencing runs against whole genomes or transcriptomes. Optimizes composite paired-read scores with splice-aware alignment, exc
Use with AI
Install the MCP server or CLI to instantly fetch Magic-BLAST documentation:
Install command
claude mcp add biocontext7 -- npx @biocontext7/mcpOr share this page: biocontext7.com/tools/magicblast
Graph-based alignment of reads to a population of genomes using hierarchical indexing — successor to TopHat and HISAT for RNA-seq alignment.
3 shared topics • 1 shared operation
Use when performing precision HLA class I typing from NGS data with OptiType. Covers read mapping to HLA reference with YARA, Integer Linear Programming allele calling at 4-digit resolution for HLA-A,
3 shared topics • 1 shared operation
Java command-line tools from the Broad Institute for manipulating high-throughput sequencing data and formats including BAM, CRAM, and VCF.
3 shared topics • 1 shared operation
Qualimap — platform-independent quality control tool for next-generation sequencing alignment data. Provides BAM QC (coverage, insert size, GC content, mapping quality), RNA-seq QC (gene body coverage
3 shared topics • 1 shared operation
Strelka2 — fast and accurate small variant caller for germline and somatic analysis. Detects SNVs and indels (up to ~49 bp) from mapped paired-end sequencing reads with tiered haplotype modeling, adap
3 shared topics