BUSCO (Benchmarking Universal Single-Copy Orthologs) — assess genome assembly, transcriptome, and proteome completeness using lineage-specific single-copy orthologs from the OrthoDB database. Reports
Use with AI
Install the MCP server or CLI to instantly fetch BUSCO documentation:
Install command
claude mcp add biocontext7 -- npx @biocontext7/mcpOr share this page: biocontext7.com/tools/busco
Ensembl Database — EMBL-EBI's comprehensive genome annotation database covering 300+ species with gene models, variants, regulatory features, and comparative genomics. Query via REST API at rest.ensem
2 shared topics • 1 shared operation
gffread -- GFF/GTF utility for filtering, converting, and extracting sequences from genome annotation files. Converts between GFF3 and GTF formats, extracts transcript (FASTA) and protein sequences fr
2 shared topics • 1 shared operation
MAKER — portable genome annotation pipeline that integrates ab initio gene predictors (SNAP, Augustus, GeneMark), protein and EST evidence alignment, and repeat masking to produce GFF3 gene models wit
2 shared topics • 1 shared operation
ncbi-genome-download is a Python CLI tool and library for bulk downloading genome sequences and associated files from NCBI RefSeq and GenBank FTP servers. Supports all taxonomic groups (bacteria, fung
2 shared topics • 1 shared operation
TransDecoder — identifies candidate coding regions within transcript sequences from de novo RNA-Seq assemblies. Provides TransDecoder.LongOrfs for extracting long open reading frames and TransDecoder.
2 shared topics • 1 shared operation