Guppy — Oxford Nanopore Technologies neural-network basecaller for converting raw nanopore signal data (FAST5/POD5) into nucleotide sequences (FASTQ/BAM). Supports GPU-accelerated basecalling, barcodi

10

Oxford Nanopore Technologies (ONT)

Oxford Nanopore Technologies (ONT) sequencing data analysis skill. Covers the end-to-end nanopore pipeline: basecalling raw POD5/FAST5 signal with Dorado, long-read alignment with Minimap2, haploid co

8

nanopore-direct-rna

Nanopore direct RNA sequencing — end-to-end analysis of native RNA reads from Oxford Nanopore devices. Basecalling with Dorado, QC with NanoPlot and NanoStat, alignment with minimap2, isoform quantifi

8

Dorado

Dorado — Oxford Nanopore's official high-performance open-source basecaller that converts raw nanopore signal (POD5/FAST5) into nucleotide sequences. Replaces Guppy as the primary ONT basecaller. Supp

8

Tool	Registry	Domain	Docs
Guppy Guppy — Oxford Nanopore Technologies neural-network basecaller for converting raw nanopore signal data (FAST5/POD5) into nucleotide sequences (FASTQ/BAM). Supports GPU-accelerated basecalling, barcodi	nanoporetech/dorado	Genomics	10
Oxford Nanopore Technologies (ONT) Oxford Nanopore Technologies (ONT) sequencing data analysis skill. Covers the end-to-end nanopore pipeline: basecalling raw POD5/FAST5 signal with Dorado, long-read alignment with Minimap2, haploid co	nanoporetech/dorado	Transcriptomics	8
nanopore-direct-rna Nanopore direct RNA sequencing — end-to-end analysis of native RNA reads from Oxford Nanopore devices. Basecalling with Dorado, QC with NanoPlot and NanoStat, alignment with minimap2, isoform quantifi	nanoporetech/dorado	Other	8
Dorado Dorado — Oxford Nanopore's official high-performance open-source basecaller that converts raw nanopore signal (POD5/FAST5) into nucleotide sequences. Replaces Guppy as the primary ONT basecaller. Supp	nanoporetech/dorado	Genomics	8

Tool	Registry	Domain	Docs
Guppy Guppy — Oxford Nanopore Technologies neural-network basecaller for converting raw nanopore signal data (FAST5/POD5) into nucleotide sequences (FASTQ/BAM). Supports GPU-accelerated basecalling, barcodi	nanoporetech/dorado	Genomics	10
Oxford Nanopore Technologies (ONT) Oxford Nanopore Technologies (ONT) sequencing data analysis skill. Covers the end-to-end nanopore pipeline: basecalling raw POD5/FAST5 signal with Dorado, long-read alignment with Minimap2, haploid co	nanoporetech/dorado	Transcriptomics	8
nanopore-direct-rna Nanopore direct RNA sequencing — end-to-end analysis of native RNA reads from Oxford Nanopore devices. Basecalling with Dorado, QC with NanoPlot and NanoStat, alignment with minimap2, isoform quantifi	nanoporetech/dorado	Other	8
Dorado Dorado — Oxford Nanopore's official high-performance open-source basecaller that converts raw nanopore signal (POD5/FAST5) into nucleotide sequences. Replaces Guppy as the primary ONT basecaller. Supp	nanoporetech/dorado	Genomics	8

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