Fastools

Use when working with actc — a Pacific Biosciences (PacBio) command-line tool that aligns CLR subreads to their corresponding CCS (Circular Consensus Sequencing) reads from the same ZMW (zero-mode wav

2 shared topics • 1 shared operation

Novoalign — high-accuracy short-read sequence aligner for Illumina and MGI platforms using full Needleman-Wunsch algorithm with affine gap penalties. Maps single-end and paired-end reads up to 950bp o

1 shared topic • 2 shared operations

PRINSEQ++ — high-performance C++ tool for quality control and preprocessing of FASTQ sequencing reads. Filters and trims reads by quality score, length, GC content, sequence complexity (DUST/entropy),

1 shared topic • 2 shared operations

Trimmomatic — flexible, multithreaded read trimming tool for Illumina sequence data. Performs adapter removal (palindrome and simple modes), quality trimming (sliding window, leading/trailing), hard c

1 shared topic • 2 shared operations

vt — C++ command-line variant tool set for manipulating VCF files. Provides variant normalization (left-alignment and trimming), multiallelic decomposition, VCF summary statistics (peek), annotation,

1 shared topic • 2 shared operations