CRABS (Creating Reference databases for Amplicon-Based Sequencing)

Use when working with the ARTIC viral amplicon sequencing command-line pipeline for Oxford Nanopore data. Covers documented `artic minion`, `artic guppyplex`, and `artic_get_models` workflows for prim

2 shared topics • 1 shared operation

Deblur is a metagenomics and microbiome tool for denoising 16S rRNA amplicon sequencing data, producing exact sequence variants (ESVs/sOTUs) by applying an error model to demultiplexed single-end read

2 shared topics • 1 shared operation

EMU (Exact Mapping-based Unambiguous) — species-level 16S rRNA taxonomic profiling for long-read sequencing data (Oxford Nanopore, PacBio). Uses an expectation-maximization algorithm to resolve multi-

2 shared topics • 1 shared operation

Use when working with MEGAN (MEtaGenome ANalyzer) or MEGAN-CE — interactive exploration and taxonomic/functional classification of metagenome sequencing data. Applies the LCA (Lowest Common Ancestor)

2 shared topics • 1 shared operation

VSEARCH — open-source, multithreaded alternative to USEARCH for amplicon and metagenomics sequence analysis. Performs dereplication, chimera detection (de novo and reference-based), OTU/ASV clustering

2 shared topics • 1 shared operation